Research topic: The Molecular Basis of the Regulation of Microglial cells activity by Ion Channels
Research methods: Fluorescence-detection Size-Exclusion Chromatography (FSEC), membrane protein purification, proteoliposome reconstitution, unnatural amino acids (UAA) incorporation, transition-ion metal Fluorescence Resonance Energy Transfer (FRET), electrophysiology, patch clamp fluorometry, x-ray crystallography, deep mutational scanning, Single molecule Total Internal Reflection Microscopy (TIRF) imaging.
Projects in the lab include:
- Identification of the molecular determinants governing the activation of Ca2+-activated chloride channels.
- Elucidate the molecular rearrangements and architecture of membrane-embedded anion conductive Chloride Intracellular Channel (CLIC).
- Explore the functional expression and physiological importance of anion channels in resting and activated microglia